COMPARATIVE STUDIES OF THE YIELD AND CHEMICAL CONSTITUENTS OF Bryophyllumpinnatum BY COLD MERCERATION AND SOHXLET EXTRACTION METHODS

Abstract

Bryophyllum pinnatum belongs to the family crassulaceae and is widely used in traditional medicine, especially in tropical areas. In the present study, extract yield and phytochemical constituents of chloroform extracts of Bryophyllum pinnatum leave prepared using two most commonly used extraction techniques known as cold maceration, and soxhlet extraction was investigated. Analysis using gas chromatography and mass spectrometry (GC/MS) was done on the extracted materials. The extract produced by the cold maceration method yielded ten (10) compounds, whereas the extract produced by the soxhlet extraction method yielded eight (8) compounds. Piperazine, 1-(2-adamantyl)-4-benzoyl, which made up the highest percentage of the 10 compounds found using the cold maceration method, had a concentration of 53.673%, while Pyrazine, 2,6-diethyl, had a concentration of 31.913% in the extract made using the soxhlet extraction method. The GCMS analysis's findings suggest that heat can change the composition of several phytochemical substances. The term thermo-liable compounds refer to those substances that are easily denatured or changed by heat. The variance in yield percentages and phytochemical compositions that has been observed is due to the employment of various extraction methods. The presence of these phytochemicals in B. Pinnatum leaves supports the use of the plants' leaves in the treatment of a variety of illnesses, including leishmaniasis, asthma, and ear pain, as several of the compounds discovered are known to have therapeutic properties.