Abstract
153Smcitrate solutions were prepared from enriched 152Sm 2O 3 which had been irradiated at 10 12n cm −2 s −1 in the University of Alberta Slowpoke reactor. 153Sm was rapidly bound (93% in 2 h) by Melanoma 2AB cells in tissue culture upon incubation in the presence of 153Smcitrate (1.9 nmol 10 6 cells). In vitro ultracentrifugation studies of 153Smcitrate solutions showed that colloid formation under incubation conditions could have been responsible in part for the uptake by cultured cells. Low uptakes (<1% in 2h) of 67Gacitrate were seen under similar conditions. 153Smcitrate injected into BDF 1 mice (Lewis lung carcinoma) and Copenhagen × Fisher rats (Dunning R3327-H prostatic tumors) was concentrated mainly in the liver, with some tumor and bone uptake. The percent of injected dose per organ for 153Sm and 67Ga in the murine and rat models respectively, 24 h after i.v. dosing, was 17.2 ± 4.7 and 2.0 ± 0.6 (tumor), 63.9 ± 7.9 and 14.4 ± 1.4 (liver) and 0.6 ± 0.1 and 1.3 ± 0.1 (blood); % injected dose g −1 femur was 6.2 ± 2.7 and 12.9 ± 2.7, respectively. Scintigrams of rats showed qualitative biodistributions similar to the quantitative mouse data obtained by dissection studies. The high hepatic uptake detracted from the otherwise superior tumor localization of 153Sm citrate when compared to 67Gacitrate in these models. The murine Lewis lung tumor index (% injected dose g −1 tumor × tumor:blood) was 303.6 for 153Smcitrate and 48.9 for 67Gacitrate, 24 h after injection.
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More From: International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology
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