Comparative Studies between Different Types of Live Infectious Bursal Disease (IBD) Vaccine Strains in Egypt

Abstract

This study was carried out to test the efficacy of different live infectious bursal disease (IBD) vaccines (intermediate; classical or intermediate plus) for controlling IBD or as it is more commonly known in Egypt as gumboro disease problems in the Egyptian poultry field. The efficacy of different living attenuated commercial vaccines were applied in ten groups of (20) Specific Pathogen Free chicks (SPF) for monitoring the immunosuppression effect. The immune response were determined in nine groups of (75) two weeks old SPF chicks “for each group” in vitro by measuring Enzyme Linked Immuno-Sorbent Assay (ELISA) and Serum Neutralization Test (SNT) titer post vaccination with estimation of bursal /body weight ratio and histopathological examination of bursa of fabricious; then in vivo by challenging birds with 10 3.5 EID50 / dose challenge IBD virus strains (variant; classical and very virulent strains). Results revealed that; protection percentages were ranged between 90%-100% in birds vaccinated with intermediate or intermediate plus IBD vaccine and between 90%-95% in birds vaccinated with invasive intermediate Bursa B2K. While birds vaccinated with classical D78 gave protection 95%-100% with highest antibody ELISA mean titer and SNT were “11344 and 1024”; respectively. This confirms that under field condition, poultry industry can be protect from gumboro disease if using commercial IBD vaccine