Comparative stability study of thymidine and (dideoxy- d- erythro-hexopyranosyl)thymine analogues monitored by capillary electrophoresis

Abstract

A capillary zone electrophoretic method was developed for monitoring the stability of hexopyranosyi analogues of thymidine. The final analytical conditions adopted were as follows: capillary, fused silica, 70 cm (62 cm to detector) × 75 μm I.D.; background electrolyte, 20 m M sodium tetraborate (pH 10.5); voltage, 30 kV; detection, UV at 262 nm; and temperature. 15°C. The performance of this analytical system is discussed. The stability study at three pH values allows to compare the stability of the analogues with that of thymidine and to investigate the influence of the configuration of the N-glycosidic bond on the rate constant of degradation.