Abstract
Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.
Highlights
Pleuropneumonia is an economically important disease causing considerable losses in the worldwide swine industry (Sassu et al, 2017)
The causative agent, Actinobacillus pleuropneumoniae, can be differentiated into 2 biovars based on the requirement for nicotinamide adenine dinucleotide (NAD-dependent biovar 1; NAD-independent biovar 2); and subsequently into 18 serovars based on surface polysaccharides, mainly capsule (Bossé et al, 2018)
Pleuropneumoniae isolates [see (Gottschalk, 2015) for a recent review], the need for high quality reference antisera limits the number of laboratories able to perform diagnostics, and even problems with cross reactivity between certain serovars are unavoidable
Summary
Pleuropneumonia is an economically important disease causing considerable losses in the worldwide swine industry (Sassu et al, 2017). The ability to discriminate between serovars is advantageous, as there are differences in geographical distribution that are not static (Gottschalk, 2015; Sassu et al, 2017), as well as differences in levels of virulence (Klitgaard et al, 2010). Accurate typing is essential for diagnosis and for tracking the emergence of serovars rarely, or not previously, reported within a geographical region. Laboratories are using molecular typing methods to more accurately and reproducibly identify A. pleuropneumoniae isolates (Gottschalk, 2015; Sassu et al, 2017).
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