Abstract
Supercritical fluid chromatographic (SFC) technique has been widely applied in the analysis, determinations and chromatographic preparations of chiral compounds in past decades due to some advantages such as fast speed, high efficiency and low consumption of organic sol⁃ vents. Herein, 24 kinds of chiral compounds (H1-H24) have been resolved on four polysaccha⁃ ride⁃coated chiral stationary phases (CSPs) by SFC and high performance liquid chromatogra⁃ phy (HPLC), separately. Taking into account their retention times and selection factors of these compounds, SFC separation showed a better efficiency than HPLC for most of the com⁃ pounds, except for some axis⁃chiral compounds. Some complementarity between SFC and HPLC separations was observed. For H15-H17, their retentions in SFC separations showed a strengthening trend with increasing carbon numbers of side alkyl chain, whereas a reverse tend⁃ ency in HPLC separation was displayed. Most interestingly, an elution order reversal of metcon⁃ azole enantiomers was found in this work. In brief, these results would provide some valuable references for the development of SFC separation methods in the future.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
