Comparative research of photosynthetic processes in selected poikilohydric organisms from Mediterranean and Central-European alpine habitats

Abstract

Dehydration-induced decrease in photosynthetic activity was investigated in five poikilohydric autotrophs using chlorophyll fluorescence parameters recorded during controlled desiccation. For the study, two representatives of mosses from alpine zone (Rhizomnium punctatum, Rhytidiadelphus squarrosus) of the Jeseníky Mts. (Czech Republic) were used. Other two experimental species were mediterranean habitats liverwort (Pellia endiviifolia) and moss (Palustriella commutata), collected from under Woodwardia radicans canopy in the Nature Reserve Valle delle Ferriere (Italy). The last species was a liverwort (Marchantia polymorpha) collected from lowland site (Brno, Moravia, Czech Republic). We investigated the relationship between relative water content (RWC) and several chlorophyll fluorescence parameters evaluating primary photochemical processes of photosynthesis, such as effective quantum yield of photosynthetic processes in photosystem II (ΦPSII), and non-photochemical quenching (qN). With desiccation from fully wet (RWC = 100%) to dry state (RWC = 0%), ΦPSII exhibited a rapid (R. punctatum) and slow decline of ΦPSII (R. squarrosus, P. endiviifolia, M. polymorpha, and P. commutata). Shapes of dehydration-response curves were species-specific. RWC0.5, i.e. the RWC at which the sample showed half of maximum ΦPSII, reflected the species-specificity. It reached 65% in desiccation sensitive (R. punctatum), 53% and 43% in semi-tolerant (P. commutata and R. squarrosus), 24% and 18% in desiccation-tolerant species (P. endiviifolia and M. polymorpha). In all experimental species, non-photochemical quenching (qN) of absorbed light energy showed high values at RWC = 100% and a slight increase with desiccation. Steady state chlorophyll fluorescence (FS) remained high during desiccation and was not correlated with ΦPSII.