Comparative radiosensitization efficiency assessment of graphene oxide and Ti3C2 MXene as 2D carbon-based nanoparticles against breast cancer cells: characterization, toxicity and mechanisms

Abstract

The efficacy of two carbon-based nanomaterials, graphene oxide (GO) and Ti3C2 MXene (MX), on the radiosensitivity of the breast cancer cells (BCCs) was investigated using clinical x-ray irradiation. The prepared GO and MX nanoparticles (NPs) were firstly characterized utilizing Fourier transform infrared, ultraviolet–visible, atomic force microscopy and transmission electron microscopy techniques and subsequently assessed in terms of their radiobiological properties. The results of the cell toxicity assay indicated that neither NPs exhibited significant cytotoxicity after 48 h incubation with BCC up to 50 µg ml−1 concentration without irradiation. The cell internalization results showed an approximately equivalent cellular uptake for both NPs after 6 h incubation with BCC. Our comparative studies with radiotherapy demonstrated that both NPs substantially increased cell proliferation inhibition and cell apoptosis of BCC under x-ray irradiation when compared to BCC treated with irradiation alone. Additionally, the 2ʹ,7ʹ-dichlorofluorescin diacetate flow cytometry results and fluorescent microscopy images revealed that both NPs remarkably increased the level of intracellular reactive oxygen species (ROS) generation in BCC under x-ray irradiation. The MX nanosheets exhibited superior radiosensitization efficiency than GO under x-ray irradiation due to its higher level of intracellular ROS generation (MX = 75.2% and GO = 65.2%). Clonogenic cell survival assay and extracted radiobiological parameters revealed that both NPs in combination with x-ray irradiation induced more lethal damage and less sublethal damage to BCC. Generally, the obtained results demonstrate that the MX NPs, as a stronger radiosensitizer than GO, could be a promising candidate for enhancing the effectiveness of radiotherapy in breast cancer treatment.