Abstract
BackgroundLaminitis is considered as the most important cause of hoof lameness in dairy cows, which causes abundant economic losses in husbandry. Through intense efforts in past decades, the etiology of laminitis is preliminarily considered to be subacute ruminal acidosis; however, the pathogenesis of laminitis needs further research. The differentially expressed proteins (DEP) were detected in plasma of healthy cows and clinical laminitis cows by two-dimensional gel electrophoresis (2-DE) and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.ResultsNineteen protein spots were differentially expressed, and 16 kinds of proteins were identified after peptide mass fingerprint search and bioinformatics analysis. Of these, 12 proteins were differentially up-regulated and 4 down-regulated. Overall, these differential proteins were involved in carbohydrate metabolism, lipids metabolism, molecular transport, immune regulation, inflammatory response, oxidative stress and so on.ConclusionsThe DEPs were closely related to the occurrence and development of laminitis and the lipid metabolic disturbance may be a new pathway to cause laminitis in dairy cows. The results provide the theory foundation for further revealing the mechanism of laminitis and screening the early diagnostic proteins and therapeutic target.
Highlights
Laminitis is considered as the most important cause of hoof lameness in dairy cows, which causes abundant economic losses in husbandry
Comparative proteomic analysis of plasma samples Scanning of 2-DE gel maps showed that several spots did not correspond to proteins, which may be due to contamination or gel impurities
The current study provides a novel report on plasma proteomic profiles of laminitis in dairy cows
Summary
Laminitis is considered as the most important cause of hoof lameness in dairy cows, which causes abundant economic losses in husbandry. The problem’s prevalence is a significant contributor to Laminitis—an inflammation of the laminar corium in the hoof wall—is a common underlying cause of lameness, accounting for 41 % of the cases of lameness identified in cows [1]. It can result in many kinds of claw horn lesions including sole hemorrhages, white line disease, and sole ulcers in dairy cattle [2], which highly affect a cow’s welfare, production, and longevity. Through intense efforts to understand the root cause of clinical
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