Abstract
Abstract Fruit fly development has been widely studied in details, understanding of “what”, “when”, “where”, “why”, and “how” many hundred thousand proteins exist in an insect cell interact and express during development at molecular level largely remained to be clarified. We conducted proteome mapping in all developmental stages of the solanum fruit fly, Bactrocera latifrons (Hendel), by comparing all ages within a stage to their 1-d-old, using 2-D gel electrophoresis and mass spectrometry. Samples of designated ages of each stage of B. latifrons were collected, analyzed, and described. A custom peptide database, derived from a publically available de novo B. latifrons transcriptome assembly was adopted for peptide identification. Identified differentially expressed proteins (DEPs) and their putative protein functions were annotated in representative SDS gel images, charts, and tables. Based on our proteomic data, we constructed a preliminary and descriptive reference proteome maps which not only provide valuable information toward a comprehensive understanding of fruit fly development, but also build a foundation for development of novel advanced fruit fly control techniques or further studies related to sterilization insect technique (SIT) and genome deletion. Any epigenetic impacts due to abiotic or biotic environmental factors will be easier to be identified, manipulated, and further led to gene editing research.
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