Comparative Proteomic Profiling Reveals Molecular Characteristics Associated with Oogenesis and Oocyte Maturation during Ovarian Development of Bactrocera dorsalis (Hendel).

Dong Wei,Zheng Zhang,Jin-Jun Wang,Yu-Wei Liu,Meng-Yi Zhang,Ran Li,Guy Smagghe

doi:10.3390/ijms18071379

Dong Wei, Zheng Zhang + Show 5 more

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https://doi.org/10.3390/ijms18071379

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Abstract

Time-dependent expression of proteins in ovary is important to understand oogenesis in insects. Here, we profiled the proteomes of developing ovaries from Bactrocera dorsalis (Hendel) to obtain information about ovarian development with particular emphasis on differentially expressed proteins (DEPs) involved in oogenesis. A total of 4838 proteins were identified with an average peptide number of 8.15 and sequence coverage of 20.79%. Quantitative proteomic analysis showed that a total of 612 and 196 proteins were differentially expressed in developing and mature ovaries, respectively. Furthermore, 153, 196 and 59 potential target proteins were highly expressed in early, vitellogenic and mature ovaries and most tested DEPs had the similar trends consistent with the respective transcriptional profiles. These proteins were abundantly expressed in pre-vitellogenic and vitellogenic stages, including tropomyosin, vitellogenin, eukaryotic translation initiation factor, heat shock protein, importin protein, vitelline membrane protein, and chorion protein. Several hormone and signal pathway related proteins were also identified during ovarian development including piRNA, notch, insulin, juvenile, and ecdysone hormone signal pathways. This is the first report of a global ovary proteome of a tephritid fruit fly, and may contribute to understanding the complicate processes of ovarian development and exploring the potentially novel pest control targets.

Highlights

The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most devastating agricultural pests worldwide due to its highly reproductive and invasive ability
90 proteins that likely regulate female reproduction were identified previously through proteomic approaches [9]. Such transcriptomes of B. dorsalis are available at NCBI, large-scale molecular analysis of reproductive proteins expressed in B. dorsalis ovary and their abundance during the ovarian development remains unknown
We observed the ovarian morphology during development at different times (Figure 1), and found that the ovary develops to the vitellogenic stage at 5 days after emergency (Figure 1C)

Summary

Introduction

The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most devastating agricultural pests worldwide due to its highly reproductive and invasive ability. Considerable efforts have been devoted to creating a male-biased reproductive tissue-specific transcriptome to identify genes involved in spermatogenesis [8] These genetic information can be served as sources for exploring new molecular targets for pest control; it is not always feasible to correlate the transcription levels of mRNAs with the synthesis of the corresponding proteins, and it is impossible to observe post-translational events such as protein modifications from gene expression studies. 90 proteins that likely regulate female reproduction were identified previously through proteomic approaches [9] Such transcriptomes of B. dorsalis are available at NCBI, large-scale molecular analysis of reproductive proteins expressed in B. dorsalis ovary and their abundance during the ovarian development remains unknown. Understanding the molecular mechanisms of ovary development and oogenesis is essential to manipulate female fertility

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