Comparative Proteomic Profiles of Typical Strain and Genetically Altered Variant of Vibrio cholerae O1, Biovar El Tor

S P Zadnova,Ya M Krasnov,T A Polunina,A V Fedorov,N V Kotova,A A Kritsky,N A Plekhanov,D V Badanin

doi:10.21055/0370-1069-2020-3-150-153

Abstract

Objective of the study was to identify the differences in the production of proteins in typical strain and genetically altered variant of V. cholerae O1, biovar El Tor.Materials and methods. Natural strains M1062 (Astrakhan, 1970) and M1509 (Moscow, 2010) were used as model strains in this work. Strains were cultivated on Luria Bertani agar at 37 °C. Electrophoresis was performed in accordance with W.K. Laemmli technique (1970), mass-spectrometric profiling – the method described by A. Shevchenko et al.Results and discussion. Mass-spectrometric scanning of cell lysates of the examined strains showed significant similarity of their proteomes (615 common proteins). The identified differences pertained to high expression of proteins in the strain M1062, participating in biosynthesis of DNA/RNA, included into “purines, pyrimidines, nucleosides” group, as well as regulatory proteins. In M1509 strain, biosynthesis of the proteins responsible for pathogenesis, adaptation under the influence of unfavorable environmental factors, included into “co-factors, vitamins, pigments” group, involved in lipid, carbohydrate, and protein metabolism, cellular processes, as well as proteins-transporters was increased. It has been suggested that the wide dissemination of El Tor genovariants is probably due to enhanced pathogenic and adaptive properties and also to the considerable transformation of cell metabolism.

Highlights

Objective of the study was to identify the differences in the production of proteins in typical strain and genetically altered variant of V. cholerae O1, biovar El Tor
The identified differences pertained to high expression of proteins in the strain M1062, participating in biosynthesis of DNA/RNA, included into “purines, pyrimidines, nucleosides” group, as well as regulatory proteins
It has been suggested that the wide dissemination of El Tor genovariants is probably due to enhanced pathogenic and adaptive properties and to the considerable transformation of cell metabolism

Summary

КРАТКИЕ СООБЩЕНИЯ

Цель работы – выявление различий в продукции белков у типичного штамма и генетически измененного варианта V. cholerae O1 биовара Эль Тор. Штаммы культивировали на агаре Луриа-Бертани при температуре 37 °C. Массспектрометрическое сканирование лизатов клеток исследуемых штаммов показало значительное сходство их протеомов (615 общих белков). Выявленные различия касались повышенной экспрессии у штамма М1062 белков, которые участвуют в биосинтезе ДНК/РНК, входящих в группу «пурины, пиримидины, нуклеозиды», а также регуляторных белков. У штамма М1509 увеличен биосинтез белков, ответственных за патогенез, адаптацию при действии неблагоприятных факторов внешней среды, входящих в группу «кофакторы, витамины, пигменты», участвующих в липидном, углеводном, белковом обменах, клеточных процессах, и белков-транспортеров. Что широкое распространение геновариантов Эль Тор, возможно, обусловлено повышением патогенных и адаптивных свойств, а также существенной перестройкой метаболизма клеток. Ключевые слова: Vibrio cholerae биовара Эль Тор, протеомное сканирование. Comparative Proteomic Profiles of Typical Strain and Genetically Altered Variant of Vibrio cholerae O1, Biovar El Tor

Brief communication

Материалы и методы

Результаты и обсуждение

Список литературы