Abstract
Acute aortic dissection (AAD) is a catastrophic cardiovascular disease with high disability and mortality due to multiple fatal complications. However, the molecular changes of the serum proteome after AAD are not very clear. Here, we performed isobaric tags for relative and absolute quantitation- (iTRAQ-) based comparative proteomic analysis to investigate the proteome profile changes after AAD by collecting plasma samples from 20 AAD patients and 20 controls. Out of the 345 identified proteins, 266 were considered as high-quality quantified proteins (95%confident peptides ≥ 2), of which 25 proteins were accumulated and 12 were reduced in AAD samples. Gene ontology enrichment analysis showed that the 25 AAD-accumulated proteins were enriched in high-density lipoprotein particles for the cellular component category and protein homodimerization acidity for the molecular function category. Protein-protein interaction network analysis showed that serum amyloid A proteins (SAAs), complement component proteins, and carboxypeptidase N catalytic chain proteins (CPNs) possessed the key nodes of the network. The expression levels of six selected AAD-accumulated proteins, B2-GP1, CPN1, F9, LBP, SAA1, and SAA2, were validated by ELISA. Moreover, ROC analysis showed that the AUCs of B2-GP1 and CPN1 were 0.808 and 0.702, respectively. Our data provide insights into molecular change profiles in proteome levels after AAD and indicate that B2-GP1 and CPN1 are potential biomarkers for AAD.
Highlights
Acute aortic dissection (AAD) is a catastrophic cardiovascular disease caused by injury of the innermost layer of the aorta, leading to vascular wall stratification by blood flows between the layers of the aortic wall [1, 2]
Our study provided new evidence for potential biomarkers for acute aortic dissection
Inclusion criteria were as follows: (1) patients with acute aortic dissection should be confirmed by the aortic computed tomography angiography (CTA) or angiography; (2) non-AAD patients should be confirmed by coronary artery angiography
Summary
Acute aortic dissection (AAD) is a catastrophic cardiovascular disease caused by injury of the innermost layer of the aorta, leading to vascular wall stratification by blood flows between the layers of the aortic wall [1, 2]. Treatment guidelines have been well established, AAD has high fatality caused by irreversible vascular damage before the patient arrives at the hospital [3]. Combined with standard imaging methods using computed tomography angiography (CTA) and magnetic resonance angiography (MRA), quick diagnosis of AAD before the patients’ arrival to the hospital using biomarkers with high sensitivity and specificity will greatly improve the survival rate and treatment guidelines [6,7,8]. All Disease Markers these potential biomarkers have disadvantages in specificity or sensitivity
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