Comparative Proteomic Analysis Reveals the Cross-Talk between the Responses Induced by H2O2 and by Long-Term Rice Black-Streaked Dwarf Virus Infection in Rice

Qiufang Xu,Tong Zhou,Haiping Ni,Feng Sun,Ying Lan,Qingqing Chen,Yijun Zhou,Vladimir N Uversky

doi:10.1371/journal.pone.0081640

Abstract

Hydrogen peroxide (H2O2) could be produced during the plant-virus compatible interaction. However, the cell responses regulated by the enhanced H2O2 in virus infected plant are largely unknown. To make clear the influence of Rice black-streaked dwarf virus (RBSDV) infection on H2O2 accumulation, we measured the content of H2O2 and found the H2O2 level was increased in rice seedlings inoculated with RBSDV. To reveal the responses initiated by the enhanced H2O2 during plant-virus interaction, the present study investigated the global proteome changes of rice under long-term RBSDV infection. Approximately 1800 protein spots were detected on two-dimensional electrophoresis (2-DE) gels. Among them, 72 spots were found differently expressed, of which 69 spots were successfully identified by MALDI-TOF/TOF-MS. Furthermore, the differentially expressed proteins induced by RBSDV infection were compared to that induced by H2O2. 19 proteins corresponding to 37 spots, which were differentially expressed under RBSDV infection, were observed differentially expressed under H2O2 stress as well. These overlapping responsive proteins are mainly related to photosynthesis, redox homeostasis, metabolism, energy pathway, and cell wall modification. The increased H2O2 in RBSDV infected plant may produce an oxidative stress, impair photosynthesis, disturb the metabolism, and eventually result in abnormal growth. The data provide a new understanding of the pivotal role of H2O2 in rice-RBSDV compatible interaction.

Highlights

Hydrogen peroxide (H2O2) is one of the most important types of reactive oxygen species (ROS) and has attracted much attention during the last decades
The results presented in this study provide the framework for further functional studies of H2O2 produced under long-term virus infection
Rice black-streaked dwarf virus (RBSDV) infection elevated the H2O2 level in rice The rice seedlings infected with RBSDV exhibited notable stunting symptoms (Figure 1A)

Summary

Introduction

Hydrogen peroxide (H2O2) is one of the most important types of reactive oxygen species (ROS) and has attracted much attention during the last decades. It is well known that ROS plays a pivotal role in the defense response of plants against pathogen [1,2]. H2O2 can act as a local signal for hypersensitive response as well as a diffusible signal for the induction of defensive genes in adjacent cells [5]. Previous studies demonstrated that H2O2 has dual functions in plant. It has been proved to modulate gene expression and participate in various processes, such as cell growth, pathogen defense, programmed cell death, hormonal responses, photosynthesis regulation, and signal transduction [6,7,8]. The production of H2O2 was considered to be a non-specific response and the function of H2O2 produced during pathogen infection was rarely studied

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Conclusion