Abstract
BackgroundExogenous 6-benzyladenine (6-BA) could improve leaf defense system activity. In order to better understand the regulation mechanism of exogenous 6-benzyladenine (6-BA) on waterlogged summer maize, three treatments including control (CK), waterlogging at the third leaf stage for 6 days (V3–6), and application of 100 mg dm− 3 6-BA after waterlogging for 6 days (V3–6-B), were employed using summer maize hybrid DengHai 605 (DH605) as the experimental material. We used a labeling liquid chromatography-based quantitative proteomics approach with tandem mass tags to determine the changes in leaf protein abundance level at the tasseling stage.ResultsWaterlogging significantly hindered plant growth and decreased the activities of SOD, POD and CAT. In addition, the activity of LOX was significantly increased after waterlogging. As a result, the content of MDA and H2O2 was significantly increased which incurred serious damages on cell membrane and cellular metabolism of summer maize. And, the leaf emergence rate, plant height and grain yield were significantly decreased by waterlogging. However, application of 6-BA effectively mitigated these adverse effects induced by waterlogging. Compared with V3–6, SOD, POD and CAT activity of V3–6-B were increased by 6.9, 12.4, and 18.5%, LOX were decreased by 13.6%. As a consequence, the contents of MDA and H2O2 in V3–6-B were decreased by 22.1 and 17.2%, respectively, compared to that of V3–6. In addition, the leaf emergence rate, plant height and grain yield were significantly increased by application of 6-BA. Based on proteomics profiling, the proteins involved in protein metabolism, ROS scavenging and fatty acid metabolism were significantly regulated by 6-BA, which suggested that application of 6-BA exaggerated the defensive response of summer maize at proteomic level.ConclusionsThese results demonstrated that 6-BA had contrastive effects on waterlogged summer maize. By regulating key proteins related to ROS scavenging and fatty acid metabolism, 6-BA effectively increased the defense system activity of waterlogged summer maize, then balanced the protein metabolism and improved the plant physiological traits and grain yield.
Highlights
Exogenous 6-benzyladenine (6-BA) could improve leaf defense system activity
Plant growth and grain yield of summer maize The field experiment results showed that waterlogging significantly impeded the plant growth by decreasing leaf emergence rate and plant height
The present study proved that 6-BA increased the defense system activity by modulating the expression of proteins related with reactive oxygen species (ROS) and fatty acid metabolism which worked together to maintain the integrity of cell membranes
Summary
Exogenous 6-benzyladenine (6-BA) could improve leaf defense system activity. In order to better understand the regulation mechanism of exogenous 6-benzyladenine (6-BA) on waterlogged summer maize, three treatments including control (CK), waterlogging at the third leaf stage for 6 days (V3–6), and application of 100 mg dm− 3 6-BA after waterlogging for 6 days (V3–6-B), were employed using summer maize hybrid DengHai 605 (DH605) as the experimental material. The spatial and temporal distribution of rainfall has tended toward extremes and adverse climatic events, including waterlogging, drought, heat injury, low temperature, and freezing damage, are expected [3]. Such extreme events have done untold damages to environment, agricultural production, and long-term prospects of economy [4,5,6,7]. During the whole life cycle of maize production in Huang-Huai-Hai Plain, the frequency of waterlogging is as high as 30%, especially during seedling to jointing and jointing to tasseling stages, which damages plant growth and increases grain yield loss significantly [9]
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