Abstract
Objective To identify the differentially expressed proteins in hepatocytes within the optimal co-cultivation system with bone marrow mesenchymal stem cells (MSCS).Methods The optimal co-cultivation of MSCS and primary hepatocytes was generated in vitro.CD44-CD45-cell population (hepatocytes) was sorted from co-culture system by flow cytometry.Protein samples were isolated by twodimensional (2D) gel electrophoresis.Bioinformatics was employed to analyze the relevance between the altered proteins.The protein expression levels of interest were confirmed by Western blotting.Results Thirty-four altered protein spots were unambiguously identified with high confidence,which are likely associated with hepatic metablic functions,cell cycle,cellular proliferation and apoptosis,as well as immune modulation.Conclusion Several differentially expressed functional proteins within co-cultured hepatocytes have been revealed by comparative proteomics,which may be associated with enhanced hepatic functionality,prolonged cellular survival,and immune modulation. Key words: Porcine hepatocytes ; Bone marrow mesenchymal stem cells ; Co-culture ; Proteome
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