Abstract
Waterlogging severely affects global agricultural production. Clarifying the regulatory mechanism of grapevine in response to waterlogging stress will help to improve the waterlogging tolerance of grapevine. In the present study, the physiological and proteomic responses of SO4 grapevine rootstock to different waterlogging tolerances were comparatively assayed. The results showed that the activities of SOD and POD first increased and then decreased, while the change trend of CAT and APX activities was the opposite. In addition, the MDA and H2O2 contents increased after waterlogging treatment, but the chlorophyll a and chlorophyll b contents decreased. A total of 5,578 grapevine proteins were identified by the use of the tandem mass tag (TMT) labeling technique. Among them, 214 (103 and 111 whose expression was upregulated and downregulated, respectively), 314 (129 and 185 whose expression was upregulated and downregulated, respectively), and 529 (248 and 281 whose expression was upregulated and downregulated, respectively) differentially expressed proteins (DEPs) were identified in T0d vs. T10d, T10d vs. T20d, and T0d vs. T20d comparison groups, respectively. Enrichment analysis showed that these DEPs were mainly involved in glutathione metabolism, carbon fixation, amino sugar and nucleotide sugar metabolism, biosynthesis of amino acids, photosynthesis, carbon metabolism, starch, and sucrose metabolism, galactose metabolism, protein processing and ribosomes. To further verify the proteomic data, the expression of corresponding genes that encode eight DEPs was confirmed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). The results of this study presented an important step toward understanding the resistance mechanisms of grapevine in response to waterlogging stress at the proteome level.
Highlights
Waterlogging is one of the major abiotic stresses that subjects plants to low-oxygen conditions and has a substantial negative influence on plant growth and development (Sairam et al, 2009)
Our results showed that waterlogging treatment decreased the expression ferredoxin (VIT_12s0035g00270), ferredoxinthioredoxin reductase (VIT_10s0071g01160 and VIT_14s0066 g01900), ferredoxin-NADP reductase (VIT_18s0001g14450), cytochrome P450 (VIT_03s0167g00190 and VIT_07s0129g 00790), and cytochrome b6-f complex iron-sulfur subunit 1 (VIT_19s0014g03850) proteins, which means that waterlogging stress may affect the photosystems in grapevine by inhibiting electron transport reactions
This study showed that waterlogging stress contributes to the expression of serine/threonine protein kinases (VIT_07s0031g03210, VIT_07s0191g00070, and VIT_05s0020g01990), which showed that these proteins may be related to grapevine tolerance to waterlogging
Summary
Waterlogging is one of the major abiotic stresses that subjects plants to low-oxygen conditions and has a substantial negative influence on plant growth and development (Sairam et al, 2009). Because of the Proteomic Analysis to Waterlogging Stress low diffusion coefficient of oxygen in water, waterlogged tissues cannot obtain enough oxygen for aerobic respiration (Van Veen et al, 2014). The lack of oxygen severely affects plants at most growth and development stages and causes changes in energy-related metabolic pathways from aerobic respiration to anaerobic fermentation (Jackson and Colmer, 2005; Xu et al, 2014; Zhou et al, 2020). Rice and grass plants adapt to waterlogging stress via rapid stem/hypocotyl elongation (Evans, 2004; Jiang et al, 2010; Liu and Jiang, 2015)
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
