Abstract
Trichosanthin (TCS) is a traditional Chinese herbal medicine used to treat some gynecological diseases. Its effective component has diverse biological functions, including antineoplastic activity. The human trophoblast cell line BeWo was chosen as an experimental model for in vitro testing of a drug screen for anticancer properties of TCS. The MTT method was used in this study to get a primary screen result. The result showed that 100 mM had the best IC50 value. Proteomics analysis was then performed for further investigation of the drug effect of TCS on the BeWo cell line. In this differential proteomic expression analysis, the total proteins extracted from the BeWo cell line and their protein expression level after the drug treatment were compared by 2DE. Then, 24 unique three-fold differentially expressed proteins (DEPs) were successfully identified by MALDI-TOF/TOF MS. Label-free proteomics was run as a complemental method for the same experimental procedure. There are two proteins that were identified in both the 2DE and label-free methods. Among those identified proteins, bioinformatics analysis showed the importance of pathway and signal transduction and gives us the potential possibility for the disease treatment hypothesis.
Highlights
TCS is an abortifacient protein purified from Tian-hua-fen, a traditional Chinese medicine obtained from the root tubers of Chinese trichosanthes
BeWo cells were cultured according to the protocol recommended by the American Type Culture Collection (ATCC) [24]
A picture of 96-well plate is attached as the Figure S1 and detailed concentration-dependent MTT result table as the Table S1
Summary
TCS is an abortifacient protein purified from Tian-hua-fen, a traditional Chinese medicine obtained from the root tubers of Chinese trichosanthes. It is a ribosome-inactivating protein with high antitumor activity [1] and endowed with multiple vital biological activities, including anti-HIV and antitumor processes [2]. TCS is a prescription drug in China for gynecological use in various diseases such as ectopic pregnancies, hydatidiform moles, chorionic epithelioma, and abortions [3]. It is normally used as a pharmaceutical material for midterm abortion. The anticancer activities of TCS are associated with the induction of specific changes of the cytoskeleton configuration by reducing the expression of tubulins [8]
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