Comparative proteomic analysis of drought and high temperature response in roots of two potato cultivars

Dominika Boguszewska-Mańkowska,Małgorzata Nykiel,Marta Gietler

doi:10.1007/s10725-020-00643-y

Abstract

A comparative analysis of drought and high temperature responsive proteins, which means to provide insight into the molecular mechanism of potato stress tolerance. In the presented study, two potato cultivars, differing in dehydration tolerance, were compared. An analysis of their morphological, physiological and root proteome related traits proved that, although water shortage, as well as high temperatures cause the dehydration of plants, the response to those stresses at the proteome level was significantly different. LC–MS/MS protein identification showed that in roots of the sensitive cultivar, in response to drought, most changes concern increased abundance of defence- and detoxification-related proteins, while in tolerant plants, significant changes in abundance of energy and carbohydrate metabolism related proteins were observed (data are available via ProteomeXchange with identifier PXD020259). Moreover, in response to high temperatures, in the sensitive cultivar, decreased abundance of proteins involved in cell energetic metabolism was detected, while in the tolerant cultivar, the majority of proteins from this group was abundant. It can be suggested that such comparative proteome analysis indicates the fine tuning metabolism as a major factor of stress tolerance of potato plants.

Highlights

The ongoing climate change associated with increased temperature and reduced rainfall negatively impacts plant growth, development and crop yield (Chaves et al 2003)
A 14-day period of withholding watering of potato plants, three weeks after the initiation of the tuberisation phase, resulted in a significant decline in the water content in leaves measured as relative water content (RWC) in both examined cultivars (Table 1)
There was no significant difference between RWC of leaves of control plants of both cultivars and those treated with high temperature (Table 1)

Summary

Introduction

The ongoing climate change associated with increased temperature and reduced rainfall negatively impacts plant growth, development and crop yield (Chaves et al 2003). The potato (Solanum tuberosum L.) is considered to be a crop species sensitive to drought (Monneveux et al 2013) and high temperatures (Levy and Veilleux 2007). Water shortages can cause a potato yield reduction of up to 79% (Binod et al 2015). Due to the climate changes, it is estimated that potential potato yield will decrease by 18 to 32% worldwide between 2040 and 2069 (Hijmans 2003). Rain shortage in 2010 in Russia led to approximately 30% yield losses on industrial potato farms (GAIN 2010), which caused significant financial losses. Mittler (2006) studies showed that the losses in US agricultural production caused by drought amounted up to approximately USD 20 trillion in the years 1980–2004, and combined with the occurrence of high temperatures they reached USD 120 trillion. It often occurs that stress caused by heat and drought critically affects potato production (Ahn et al 2004)

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Discussion

Conclusion