Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China.

Hua Yu,Haixia Wang,Haijun Jin,Jia Zhang

doi:10.3390/molecules25163605

Abstract

Dipsacus asperoides is a kind of Chinese herbal medicine with beneficial health properties. To date, the quality of D. asperoides from different habitats has shown significant differences. However, the molecular differences in D. asperoides from different habitats are still unknown. The aim of this study was to investigate the differences in protein levels of D. asperoides from different habitats. Isobaric tags for relative and absolute quantification (iTRAQ) and 2DLC/MS/MS were used to detect statistically significant changes in D. asperoides from different habitats. Through proteomic analysis, a total of 2149 proteins were identified, of which 42 important differentially expressed proteins were screened. Through in-depth analysis of differential proteins, the protein metabolism energy and carbohydrate metabolism of D. asperoides from Hubei Province were strong, but their antioxidant capacity was weak. We found that three proteins, UTP-glucose-1-phosphate uridylyltransferase, allene oxide cyclase, and isopentyl diphosphate isomerase 2, may be the key proteins involved in dipsacus saponin VI synthesis. Eight proteins were found in D. asperoides in response to environmental stress from different habitats. Quantitative real-time PCR analysis confirmed the accuracy and authenticity of the proteomic analysis. The results of this study may provide the basic information for exploring the cause of differences in secondary metabolites in different habitats of D. asperoides and the protein mechanism governing differences in quality.

Highlights

Dipsacus asperoides is a traditional Chinese medicinal plant
The use of proteomics technology is conducive to revealing the reasons the differences in the quality of Chinese herbal medicines in different habitats at the molecular level
(1) Through the analysis of the differential proteins, we found that the protein, energy, and carbon metabolism of D. asperoides from Hubei province were robust

Summary

Introduction

Dipsacus asperoides is a traditional Chinese medicinal plant. The root of this plant is generally used as a medicine and is frequently prescribed by Chinese doctors for the treatment of back pain, limb paralysis, flutter trauma, tendon injuries, and fractures [1]. D. asperoides is widely distributed in southwestern China. Some large-scale cultivation bases for D. asperoides were established in Hefeng city, Hubei province (29◦ 820 58” N, 110◦ 580 22” E, H group); Xichang city, Sichuan province (28◦ 40 4” N, 102◦ 80 36” E, S group); Xifeng city, Guizhou province (27◦ 100 73” N, 106◦ 740 38” E, G group); and Jianchuan city, Yunnan province (26◦ 310 91” N, 99◦ 850 24” E, Y group), China. Hefeng city of Hubei province is the main producing area of D. asperoides, and the other three areas are emerging producing areas

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