Abstract
ABSTRACTA comparative analysis of egg yolk proteome of three major poultry species, namely, chicken, duck, and quail, was carried out with two-dimensional (2D) gel electrophoresis patterns and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. Proteins identified from these three poultry species shared high degrees of sequence and structure homology, which might be related to the bird’s adaptability under different environmental stresses. Few specific proteins were found in this study. Comparative 2D gel electrophoresis patterns of chicken, duck, and quail revealed that some protein-specific regions on gels could be used for authentic identification of poultry eggs. These findings could provide a fundamental understanding of different poultry egg protein profiles and showed us some new sights in egg yolk nutrients related to certain properties and functions.
Highlights
Egg yolk, well known for its high value [1,2,3], can provide almost all essential nutrients for embryo development, but may elicit specific biological functions
Additional sequence homology analysis was with the Basic Local Alignment Search Tool (BLAST)
All egg yolk protein spots on the 2D gels of these three poultry species were subsequently identified by MALDI-TOF MS/MS
Summary
Well known for its high value [1,2,3], can provide almost all essential nutrients for embryo development, but may elicit specific biological functions. It consists of 17% protein, 33% lipid, and 48% water. Various avian species may show differential egg protein patterns due to the adaptation under varied environments and evolution. As three most consumed avian eggs all over the world, chicken, quail, and duck eggs might have different egg protein patterns due to the adaptation under varied environments and evolution. According to the phylogenetic analysis with whole mitochondrial genome[10] and egg white proteome[11], Japanese quail (Coturnix japonica) and chicken had a much closer relationship
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