Abstract
Lung cancer is the leading cause of cancer‐related deaths in both men and women. Non‐small cell lung cancer (NSCLC) represents ~85% of lung cancer cases. Understanding how NSCLC cells differ from normal lung cells can help divulge the underlying causes of this disease. In this study, we analyze the protein expression pattern of an isogenic pair of normal (HBEC30KT) and NSCLC (HCC 4017) lung cell line. iTRAQ‐based quantitative differential MALDI TOF MS/MS was performed on eight cancer (NSCLC) samples and eight normal controls. We have identified 1465 proteins, 282 of which were differentially expressed with 1.3‐ fold change, with 119 up‐regulated and 163 down‐regulated. The protein expression pattern was verified by western blot for some randomly selected proteins. The up‐regulated proteins are involved in various functions including ribosome function, protein translation, mitochondrial functions and so on. However, among the down‐regulated proteins, most of them are involved in cytoskeleton and cell motility. The predicted cytoskeletal structures were also examined; as predicted from proteomic data, the cancer cell line showed thin and short actin stress fibers with reduced focal adhesion.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
