Comparative proteomic analyses of Asian cotton ovules with attached fibers in the early stages of fiber elongation process.

Bing Zhang,Jue Hu,Di Miao,Jin-Yuan Liu,Shao-Jun Du

doi:10.1186/s12953-016-0101-1

Abstract

BackgroundPlenty of proteomic studies were performed to characterize the allotetraploid upland cotton fiber elongation process, whereas little is known about the elongating diploid cotton fiber proteome.MethodsIn this study, we used a two-dimensional electrophoresis-based comparative proteomic approach to profile dynamic proteomes of diploid Asian cotton ovules with attached fibers in the early stages of fiber elongation process. One-way ANOVA and Student-Newman-Keuls test were used to find the differentially displayed protein (DDP) spots.ResultsA total of 55 protein spots were found having different abundance ranging from 1 to 9 days post-anthesis (DPA) in a two-day interval. These 55 DDP spots were all successfully identified using high-resolution mass spectrometric analyses. Gene ontology analyses revealed that proteoforms involved in energy/carbohydrate metabolism, redox homeostasis, and protein metabolism are the most abundant. In addition, orthologues of the 13 DDP spots were also found in differential proteome of allotetraploid elongating cotton fibers, suggesting their possible essential roles in fiber elongation process.ConclusionsOur results not only revealed the dynamic proteome change of diploid Asian cotton fiber and ovule during early stages of fiber elongation process but also provided valuable resource for future studies on the molecular mechanism how the polyploidization improves the trait of fiber length.Electronic supplementary materialThe online version of this article (doi:10.1186/s12953-016-0101-1) contains supplementary material, which is available to authorized users.

Highlights

Plenty of proteomic studies were performed to characterize the allotetraploid upland cotton fiber elongation process, whereas little is known about the elongating diploid cotton fiber proteome
Our results revealed the dynamic proteome change of diploid Asian cotton fiber and ovule during early stages of fiber elongation process and provided valuable resource for future studies on the molecular mechanism how the polyploidization improves the trait of fiber length
Another study that compared the proteomic profiles of wild-type and fuzzless-lintless mutant upland cotton (G. hirsutum cultivar Xu142) fibers at 10 days post-anthesis (DPA) identified 104 displayed protein (DDP) spots, providing evidence that pectin synthesis is imperative for fiber elongation [9]

Summary

Introduction

Plenty of proteomic studies were performed to characterize the allotetraploid upland cotton fiber elongation process, whereas little is known about the elongating diploid cotton fiber proteome. Another study that compared the proteomic profiles of wild-type and fuzzless-lintless mutant upland cotton (G. hirsutum cultivar Xu142) fibers at 10 DPA identified 104 DDP spots, providing evidence that pectin synthesis is imperative for fiber elongation [9]. In another 2-DE-based comparative proteomic analysis, a total of 235 protein spots were found having different abundance during the entire elongation process at five distinct time points: 5, 10, 15, 20 and 25 DPA of upland cotton (G. hirsutum cultivar CRI 35) [10]. Because most of these proteomic studies focused on the midst and late stage of fiber elongation process, the early events occur in the fiber elongation process are still poorly characterized at the proteome level

Methods

Results

Conclusion