Abstract
BackgroundThe molecular mechanism underlying broiler fat deposition is still poorly understood.MethodCurrently, we used two-dimensional gel electrophoresis (2DE) to identify differentially expressed proteins in abdominal adipose tissues of birds at 4 week of age derived from Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF).ResultsThirteen differentially expressed protein spots were screened out and identified by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The protein spots were matched to thirteen proteins by searching against the NCBInr database. These identified proteins were apolipoprotein A-I (Apo A-I), cytokeratin otokeratin, ATP synthase subunit alpha, peptidyl-prolyl cis-trans isomerase FKBP4 (PPIase FKBP4), aspartate aminotransferase, carbonic anhydrase II (CA-II), prostaglandin-H2 D-isomerase precursor, fibrinogen alpha chain, lamin-A (LMNA), superoxide dismutase [Mn] (MnSOD), heat shock protein beta-1 (HSPβ1) and two predicted proteins. These differentially expressed proteins are involved mainly in lipid metabolism, amino acid metabolism, signal transduction, energy conversion, antioxidant, and cytoskeleton. Differential expression of Apo A-I, PPIase FKBP4, and cytokeratin otokeratin proteins were further confirmed by Western blot analysis. Quantitative real-time RT-PCR analyses showed that, of these 13 differentially expressed proteins, only PPIase FKBP4 and cytokeratin otokeratin were differentially expressed at mRNA level between the two lines.ConclusionsOur results have provided further information for understanding the basic genetics control of growth and development of broiler adipose tissue.
Highlights
The molecular mechanism underlying broiler fat deposition is still poorly understood
Based on the biological process in which they are involved, these differentially expressed proteins could be classified into six categories: lipid metabolism (Apo A-I and prostaglandin-H2 D-isomerase precursor), amino acid metabolism, signal transduction, energy conversion (ATP synthase subunit alpha), antioxidant (HSPβ1, PPIase FKBP4 and MnSOD), and cytoskeleton (LMNA and cytokeratin otokeratin)
Association studies showed that a single nucleotide polymorphism (SNP) upstream of the ATG initiation codon of the chicken apolipoprotein A-I (Apo A-I) gene was associated with abdominal fat weight and abdominal fat percentage [22]
Summary
The molecular mechanism underlying broiler fat deposition is still poorly understood. Controlling fat Adipose tissue serves as a fat storage site, and as an endocrine organ that plays roles in a wide range of cellular processes including lipid metabolism and glucose homeostasis [2]. Abdominal adipose tissue is the main tissue of body fat accumulation, accounting for about 22 % of total body fat [3]. To control broiler fat deposition, it is necessary to understand gene expression and its regulation during adipose tissue development. Gene expression profiling of chicken abdominal adipose tissue has been performed, and a number of differentially expressed genes have been identified between fat and lean chickens [4,5,6,7,8].
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