Comparative Profiling of mRNA and microRNA Expression in Human Mesenchymal Stem Cells Derived from Adult Adipose and Lipoma Tissues

Abstract

Human mesenchymal stem cells have been isolated from adult adipose tissue and lipoma that is a benign neo- plasm of normal fat cells. The lipoma-derived LM6 cells exhibited a higher cumulative population doubling levels as compared with the adipose-derived AM3 cells. The expression profiles of both mRNAs and microRNAs (miRNAs) from AM3 and LM6 cells were found to exhibit considerable similarities except that miRNAs miR-99a and miR-152 were abundantly expressed in AM3, but absent in LM6 cells. The abundantly differentially expressed genes HAS2, VNN1, SLC16A6 and COL11A1 in LM6 cells were shown to be targets of miRNAs miR-99a and/or miR-152. These highly up- regulated miRNA target genes, as well as several other abundantly differentially expressed genes such as sushi domain containing 2, keratin associated proteins and tumor necrosis factor family, may explain a higher proliferation potential in LM6 cells compared with AM3 cells.