Abstract
To explore if the regulation at post-transcriptional level of follicular phase (Fols) to luteal phase (Luts) transition occurs in the ovaries of Anhuai goats, the differentially expressed microRNAs (miRNAs) of ovaries in the Fols and Luts were analyzed using Solexa sequencing in the study. In total, 320 known miRNAs were co-expressed in the two phases, 339 and 353 known miRNAs were expressed in the ovary in the Fols and Luts, respectively. In addition, 45 novel miRNAs were co-expressed in the two phases, 70 and 94 novel miRNAs were expressed in the ovary in the Fols and Luts, respectively. Let-7f was the highest expressed significantly different known miRNA in the two phases, and mir-159 was the highest expressed significantly different novel miRNA in the two phases, which may participate in the follicular-luteal transition of Anhuai goats. GO annotation and KEGG pathway analysis were applied to analyze the target genes of differentially expressed miRNAs detected in the two phases. The results will help to further understand the role of miRNAs in the regulation of follicular to luteal transition in goat ovaries.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-2902-1) contains supplementary material, which is available to authorized users.
Highlights
Ovarian activities are vital to successful reproduction and maintaining reproductive efficiency in mammals
It has been reported that the transition of follicular and luteal cell was mainly controlled by follicle-stimulating hormone and luteinizing hormone
Zhu et al SpringerPlus (2016) 5:1233 on specific breeds of mice, the roles of bone morphogenetic proteins (BMPs), activins, Smads, Wnt signaling, and aspects of the follicle-stimulating hormone (FSH) and luteinizing hormone (LH) signaling cascades are emphasized in the regulation of cyclic ovarian activity (Puglisi et al 2004; Tomizuka et al 2008)
Summary
Ovarian activities are vital to successful reproduction and maintaining reproductive efficiency in mammals. It has been reported that the transition of follicular and luteal cell was mainly controlled by follicle-stimulating hormone and luteinizing hormone. Gonadotropin-releasing hormone (GnRH) is released from the hypothalamus and controls the release of the follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary gland which induce ovarian steroid secretion that impacts the hypothalamus and pituitary through a feedback mechanism (Richards and Pangas 2010a). The regulation of follicular and luteal cell function in rodents has been described in transcriptional level (Richards 2001; Richards and Pangas 2010b). The regulation mechanism of Fols to Luts transition on the post-transcriptional is limited
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