Comparative phytochemical and biological studies of lipoidal matter of Ipomoea tricolor (Cav.) and Ipomoea fistulosa (Mart. Ex Choisy ) growing in Egypt

Abstract

GC/MS of unsaponifiable matter (USM) of Ipomoea tricolor (Cav.) and Ipomoea fistulosa (Mart. Ex Choisy) leaves were characterized by the identification of 22 and 33 compounds represented (95.88 and 91.82 %), respectively. It composed mainly of hydrocarbons and oxygenated compounds. The GC/MS of fatty acids methyl ester (FAME) led to the identification of 8 and 10 compounds for I. tricolor and I. fistulosa, respectively. Linolenic and palmatic acids were found as the predominant fatty acids. On the other hand, the petroleum erher extract of I. tricolor was fractionated on column chromatography to yield major compounds; 5-phenyldecane and 4-phenyldecane, as well as, triterpenes including β-amyrin, lupeol and simiarenol, in addition to α- amyrin, β-sitosterol, stigmasterol and α- amyrin acetate which isolated for the first time from this species. The petroleum ether extracts of I. tricolor and I. fistulosa showed a significant increase in DPPH inhibition activity at 0.01 and 0.05 ug/ml, respectively. Furthermore, I. tricolor extract exhibited a significant increase in cholinesterase inhibition activity at concentration 20 ug/ml and a significant anti-inflammatory activity with IC50 117.4µg/ml.