Abstract
A family of protein constructs has been designed to quantitatively measure changes in either macromolecular crowding or ionic strength in vivo or in vitro. These hetero-FRET protein constructs consist of the donor mCerulean3, a linker that varies with flexibility and either charged or neutral alpha helical regions, and the acceptor mCitrine. The charge of the alpha helix and the linker's rigidity determines whether it is a crowding or ionic strength sensor and its sensitivity to the environment.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
