Comparative Non Participating Transcriptome Analysis Response to Low Phosphorus by CmPht1;2 in Chrysanthemum

Abstract

Chrysanthemum morifolium Ramat is one of the four major cut flowers in the world. Pht1 family is focus on the uptake and transport of phosphate in plants. In our previous studies, CmPht1;2 overexpression line (Oe1) had higher phosphate contents both in roots and shoots, and its root development was significantly enhanced than wild type (WT) at low phosphorus conditions in chrysanthemum. Metabolomics analysis showed that several metabolites had a change in pyruvate metabolism and tricarboxylic acid (TCA) cycle pathway. To explore the gene difference expression and the change of metabolic pathway between CmPht1;2-Oe1 and WT, we conducted the transcriptome analysis. A total of 617,681 and 207,271 unigenes were obtained from roots and shoots, respectively. They were classified into biological process, cellular component and molecular function by Gene Ontology (GO). In addition, 450 different expression genes (DEGs) were found in the roots after 2 d treatment, and 1,787 DEGs were identified in shoots after 7 d treatment under LP condition between Oe1 and WT. From the top 20 pathways of DEGs assigned by Kyoto Encyclopedia of Genes and Genomes (KEGG), TCA cycle and pyruvate metabolism pathways mostly affected by overexpression of CmPht1;2 attracted our attention. This research will be helpful for elucidating the mechanism of effects by CmPht1;2 overexpression on growth, development and stress tolerance.