Abstract
A novel biological model was created for the comparison of grapevine embryogenic cells (EC) and nonembryogenic cells (NEC) sharing a common genetic background but distinct phenotypes, when cultured on their respective most appropriate media. Cytological characterization, 1H‐NMR analysis of intracellular metabolites, and glycolytic enzyme activities provided evidence for the marked metabolic differences between EC and NEC. The EC were characterized by a moderate and organized cell proliferation, coupled with a low flux through glycolysis, high capacity of phosphoenolpyruvate carboxylase and glucokinase, and high oxygen consumption. The NEC displayed strong anarchic growth, and their high rate of glycolysis due to the low energetic efficiency of the fermentative metabolism is confirmed by increased enolase capacity and low oxygen consumption.
Highlights
To cite this version: Jonathan Parrilla, Cécile Gaillard, Jérémy Verbeke, Mickael Maucourt, Radoslav Aleksandrov, et al
A novel biological model was created for the comparison of grapevine embryogenic cells (EC) and nonembryogenic cells (NEC) sharing a common genetic background but distinct phenotypes, when cultured on their respective most appropriate media
The NEC suspension was initiated using stem fragments of in vitro plants regenerated from the EC and, after its establishment, was subcultured every 2 weeks by transferring 1 mL packed cell volume (PCV) in 25 mL of fresh Gamborg medium complemented with sucrose (20 gÁLÀ1), 1-naphthalene-acetic acid (NAA) (0.18 mgÁLÀ1), and 6-benzylaminopurine (BAP) (1 mgÁLÀ1)
Summary
To cite this version: Jonathan Parrilla, Cécile Gaillard, Jérémy Verbeke, Mickael Maucourt, Radoslav Aleksandrov, et al. Comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells. HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. The EC were characterized by a moderate and organized cell proliferation, coupled with a low flux through glycolysis, high capacity of phosphoenolpyruvate carboxylase and glucokinase, and high oxygen consumption. The NEC displayed strong anarchic growth, and their high rate of glycolysis due to the low energetic efficiency of the fermentative metabolism is confirmed by increased enolase capacity and low oxygen consumption
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