Abstract
BackgroundDendrobium officinale Kimura et Migo, a member of the genus Dendrobium, is a traditional Chinese medicine with high commercial value. The positive roles of UV-B radiation on active ingredient metabolism in various medicinal plants have been studied. However, the metabolic responses of D. officinale stems to UV-B treatment is largely unknown.MethodsAn untargeted metabolomics method was used to investigate the metabolic variations in D. officinale stems between the control and UV-B treatments.ResultsIn total, 3,655 annotated metabolites, including 640 up- and 783 down-regulated metabolites, were identified and grouped into various primary metabolic categories. Then, a number of metabolites involved in the polysaccharide, alkaloid and flavonoid biosynthesis pathways were identified. For polysaccharide biosynthesis, several intermediate products, such as pyruvate, secologanate, tryptophan and secologanin, were significantly up-regulated by the UV-B treatment. For polysaccharide biosynthesis, many key fundamental building blocks, from the glycolysis, starch and sucrose metabolism, and fructose and mannose metabolism pathways, were induced by the UV-B treatment. For flavonoid metabolism, accumulations of several intermediate products of chalcone synthase, chalcone isomerase and flavanone 3-hydroxylase were affected by the UV-B treatment, indicating an involvement of UV-B in flavonoid biosynthesis. The UV-B induced accumulation of polysaccharides, alkaloids and flavonoids was confirmed by HPLC analysis. Our study will help to understand the effects of UV-B on the accumulation of active ingredients in D. officinale.
Highlights
Dendrobium officinale, an important member of the Dendrobium genus, is greatly prized medicinal herb in East Asian countries (Meng et al, 2016)
1403 metabolites were grouped into 10 primary metabolic categories, including alkaloids (176 metabolites), amino acids (330 metabolites), carotenoids (17 metabolites), lipids (102 metabolites), flavonoids (169 metabolites), hormones (52 metabolites), phenylpropanoids (112 metabolites), saccharides (168 metabolites), terpenoids (267 metabolites), and vitamins (10 metabolites) (Fig. 1D and Table S3)
Our results suggested an essential role of UV-B in the accumulation of building materials of polysaccharide biosynthesis
Summary
Dendrobium officinale, an important member of the Dendrobium genus, is greatly prized medicinal herb in East Asian countries (Meng et al, 2016). Comparative metabolomic analyses of Dendrobium officinale Kimura et Migo responding to UV-B radiation reveal variations in the metabolisms associated with its bioactive ingredients. Dendrobium officinale Kimura et Migo, a member of the genus Dendrobium, is a traditional Chinese medicine with high commercial value. The positive roles of UV-B radiation on active ingredient metabolism in various medicinal plants have been studied. The metabolic responses of D. officinale stems to UV-B treatment is largely unknown. An untargeted metabolomics method was used to investigate the metabolic variations in D. officinale stems between the control and UV-B treatments. A number of metabolites involved in the polysaccharide, alkaloid and flavonoid biosynthesis pathways were identified. Accumulations of several intermediate products of chalcone synthase, chalcone isomerase and flavanone 3-hydroxylase were affected by the UV-B treatment, indicating an involvement of UV-B in flavonoid biosynthesis. Our study will help to understand the effects of UV-B on the accumulation of active ingredients in D. officinale
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