Comparative mapping of quantitative trait loci for Fusarium head blight resistance and anther retention in the winter wheat population Capo× Arina.

Maria Buerstmayr,Hermann Buerstmayr

doi:10.1007/s00122-015-2527-8

Abstract

Key messageFusarium resistance in Arina is highly quantitative and governed by multiple small effect QTL. Anther retention has a high correlation with FHB susceptibility and appears a practicable indirect selection target for enhancing FHB resistance.The Swiss winter wheat cultivar Arina possesses a high resistance level constituted by a number of small to medium effect QTL reported from three independent mapping populations. Yet these overlap only for one resistance QTL on the long arm of chromosome 1B. The present study characterized Fusarium head blight (FHB) resistance in a population of 171 recombinant inbred lines from a cross between Arina (resistant) and Capo (moderately resistant). The population was evaluated for FHB resistance under field conditions for 3 years. Additionally, we phenotyped anther retention, plant height and flowering date to analyze their association with resistance. Lines with a low proportion of retained anthers after flowering and tall plants were significantly less diseased, while flowering date had no association with FHB severity. QTL analysis identified eight small to medium effect QTL for FHB severity, of which QTL on 1BS, 3B, 4AL and 6BL likely correspond to resistance alleles already detected in previously studied Arina populations. QTL for anther retention mapped to 4AL, 6BL and 5AS. Notably, QTL on 4AL and 6BL overlapped with QTL for FHB severity. A single small effect QTL for plant height was detected on 5AS and no QTL was identified for flowering date. Genotypes having three or four resistance alleles in combination showed a good resistance level, indicating pyramiding resistance QTL as a powerful approach for breeding resistant cultivars. Selection for rapid and complete anther extrusion appears promising as an indirect selection criterion for enhancing FHB resistance.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-015-2527-8) contains supplementary material, which is available to authorized users.

Highlights

Fusarium head blight (FHB) of wheat, predominantly caused by Fusarium graminearum and Fusarium culmorum, is a serious threat for wheat production in temperate regions throughout the world (McMullen et al 1997)
Transformed area under the disease progress curve (AUDPC) data were used as measure for FHB severity in this study, while %FHB diseased spikelets and AUDPC are not shown in the subsequent statistical analyses presented here
Transgressive segregation for FHB severity and for anther retention was observed in all experiments

Summary

Introduction

Fusarium head blight (FHB) of wheat, predominantly caused by Fusarium graminearum and Fusarium culmorum, is a serious threat for wheat production in temperate regions throughout the world (McMullen et al 1997). This destructive disease leads to losses in yield and quality (Bai and Shaner 1994) and to contamination with mycotoxins. Resistance to initial infection (type 1) and resistance to spread of the pathogen within the spike (type 2) were first described by Schroeder and Christensen (1963) and since investigated in many FHB studies.

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