Comparative Mapping of Mouse and Rat Chromosomes by Fluorescencein SituHybridization

Abstract

Comparative fluorescencein situhybridization mapping using DNA libraries from flow-sorted mouse chromosomes and region-specific mouse BAC clones on rat chromosomes reveals chromosomal homologies between mouse (Mus musculus,MMU) and rat (Rattus norvegicus,RNO). Each of the MMU 2, 3, 4, 6, 7, 9, 12, 14, 15, 16, 18, 19, and X chromosomes paints only a single rat chromosome or chromosome segment and, thus, the chromosomes are largely conserved between the two species. In contrast, the painting probes for MMU chromosomes 1, 5, 8, 10, 11, 13, and 17 produce split hybridization signals in the rat, disclosing evolutionary chromosome rearrangements. Comparative mapping data delineate several large linkage groups on RNO 1, 2, 4, 7, and 14 that are conserved in human but diverged in the mouse. On the other hand, there are linkage groups in the mouse, i.e., on MMU 1, 8, 10, and 11, that are disrupted in both rat and human. In addition, we have hybridized probes forNap2, p57, Igf2, H19,andSh3d2cfrom MMU 7 to RNO 1q and found the orientation of the imprinting gene cluster andSh3d2cto be the same in mouse and rat. Hybridization of rat genomic DNA shows blocks of (rat-specific) repetitive sequences in the pericentromeric region of RNO chromosomes 3–5, 7–13, and 20; on the short arms of RNO chromosomes 3, 12, and 13; and on the entire Y chromosome.