Comparative Mapping in thebeige–satinRegion of Mouse Chromosome 13

Abstract

The proximal end of mouse chromosome (Chr) 13 contains regions conserved on human chromosomes 1q42–q44, 6p23–p21, and 7p22–p13. This region also contains mutations that may be models for human disease, includingbeige(human Chediak–Higashi syndrome). An interspecific backcross of SB/Le andMus spretusmice was used to generate a molecular genetic linkage map of mouse chromosome 13 with an emphasis on the proximal region includingbeige(bg) andsatin(sa). This map provides the gene order of the two phenotypic markersbgandsarelative to restriction fragment length polymorphisms and simple sequence length polymorphisms in 131 backcross animals. In parallel, we have created a physical map of the region usingNidogen(Nid) as a molecular starting point for cloning a YAC contig that was used to identify thebeigegene. The physical map provides the fine-structure order of genes and anonymous DNA fragments that was not resolved by the genetic linkage mapping. The results show that thebgregion of mouse Chr 13 is highly conserved on human Chr 1q42–q44 and provide a starting point for a complete functional analysis of the entirebg–sainterval.