Comparative larval morphology and DNA barcodes of Kamimuria petasus Chen and Neoperla tuberculata Wu (Plecoptera: Perlidae)

Abstract

The larval morphology of Perlidae is relatively understudied compared to that of their adult counterparts, making it challenging to identify species using traditional morphological methods. In this study, we describe and illustrate the larvae of two stonefly species, Kamimuria petasus Chen, 2019 and Neoperla tuberculata Wu, 1937, based on specimens collected from China. Despite sharing a close resemblance in external morphology, the larvae of K. petasus and N. tuberculata can be distinguished based on their color pattern, chaetotaxy, and details of mouthparts. In addition, we generated and compared two DNA barcoding sequences for these two larvae. The analysis revealed that while the two species share some similar sequences, they exhibit high sequence divergence, which can be used to aid in the identification of the two different species based on immature material. The larval morphology and DNA barcodes provided in this study will supplement the classification of Perlidae larvae, enabling their more accurate and reliable identification in both full-evidence phylogenetic studies and bio-monitoring practices in the field.