Abstract
The effect of ALA treatment on protochlorophyll(ide) and phytochrome content in etiolated seedlings of barley (wild type and albostrians mutant), cucumber and cress was followed by in situ low-temperature (77–85 K) fluorescence spectroscopy. It was found that the treatment does not exert an effect on the phytochrome concentration in stems of cucumber and cress and in barley coleoptiles and that the phytochrome content is almost identical in wild type barley and the albostrians mutant. At the same time, the concentration of protochlorophyll(ide) exhibiting a fluorescence emission at 635 nm increased 10- to 100-fold in the ALA-treated samples. No increase was observed in the aggregated form of protochlorophyll(ide) enzyme complex with fluorescence emission at 655 nm. Taking into consideration that phytochrome and photochemically active protochlorophyllide are chromophore-protein complexes, it is suggested that the main regulatory factor for their content in the cell is the concentration of their respective apoprotein component. ALA-treatment can lead to an increase in the free tetrapyrrole concentration, but does not influence the content in apoprotein and thus the amount of chromophore-protein complexes.
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