Comparative investigations of the cellular and subcellular distribution of brain creatine kinase and [formula omitted] activity of cichlid fish and dwarf hamster

Abstract

Cellular (=total homogenate) and subcellular fractions (P1a-pellet, cytosolic fraction SN3, P4-pellet, P8-pellet, synaptosomal and synaptic membrane fraction) of brain tissue of the cichlid fish Oreochromis mossambicus and the Djungarian dwarf hamster Phodopus sungorus were collected and the distribution of creatine kinase (CK) and different Ca 2+−Mg 2+-ATPase activities in each fraction were studied. The data obtained from cellular as well as from subcellular fractions indicate less CK, Ca 2+- and ( Ca 2+−Mg 2+ )-ATPase activities in cichlid fish than in hamster brain. The dwarf hamster expressed higher enzyme activity in the brain as compared to the cichlid fish only in regard to the Mg 2+-ATPase activity and with the exception of synaptic membranes.