Comparative investigation on Camellia Sinensis mediated green synthesis of Ag and Ag/GO nanocomposites for its anticancer and antibacterial efficacy

Abstract

In the present work, Ag nanoparticles and Ag/GO nanocomposites were synthesized using the chemical reduction method with green tea leaf (Camellia Sinensis) extract as a reducing agent. Using UV-visible spectral analysis the formation of AgNPs was confirmed by the presence of strong absorption peaks appearing at 425 nm. In the case of Ag/GO nanocomposites the absorption peak shifted towards a higher wavelength of 430 nm indicating red shift. The XRD pattern revealed the structure of Ag and Ag/GO with crystallite size of 35 nm and 42 nm. FTIR analysis was carried out to know the functional group present in the samples. SEM and TEM analysis were carried out to unveil the morphology of the samples. Silver nanoparticles exhibit spherical morphology with less agglomeration whereas GO sheets were formed in which AgNPs were dispersed properly all over the surface. To know the antibacterial and anticancer efficacy Well diffusion assay and MTT assay were used. Both the samples showed potent antibacterial activity against both gram positive and gram negative bacterial strains. AgNPs and Ag/GO treated MCF-7 cell lines showed a decrease in cell viability as the concentration increased. In both studies AgNPs showed better results compared to Ag/GO which is due to the role of GO in controlling the stability of AgNPs.