Abstract
The increasing output of wastewater and landfill leachate during the rapid urbanization exacerbated the human health risks induced by antibiotic resistance genes(ARGs). For further insight into antibiotic resistance genes(ARGs) of wastewater (adding leachate before treatment) and landfill leachate, high-throughput qPCR technique was used to investigate their abundance and diversities. A total of 187 unique ARGs were detected in wastewater, which was significantly higher than that in leachate (39 unique ARGs were detected)(P<0.05). A total of 119 antibiotic resistance genes were significantly enriched in wastewater compared with the landfill leachate (P<0.05), especially the tnpA-04 and blaVEB, which belongs to mobile gene elements(MGEs) and β-Lactamase, increased by 3338-fold and 1061-fold, respectively. Our results showed that the profile of ARGs was significantly different between the wastewater and leachate, and the wastewater was the important reservoir of ARGs.
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