Comparative In vitro Anti Inflammatory Study on Alpha Amyrin Isolated from Bark Extracts of Ficus benghalensis. Linn and Alstonia boonei. De Wild

Abstract

Background Ficus benghalensis and Alstonia boonei are two good sources of triterpenes like alpha amyrin and other bioactive compounds possessing anti-inflammatory activity.Objective The present study was conducted to compare the potential in vitro anti-inflammatory activity of alpha amyrin isolated from methanolic stem bark extracts of Ficus benghalensis Linn Fb and Alstonia boonei. De Wild Ab and to identify a better source for isolation of alpha amyrin.Methods The compound alpha amyrin was identified by Thin layer chromatography TLC method followed by Liquid chromatography mass spectrometry LCMS and Fourier transform infrared FTIR studies of the extracts and with High performance liquid chromatography HPLC determination. Furthermore the compound was studied for its in vitro anti-inflammatory effect by testing its role on inhibition of albumin denaturation anti-proteinase and anti-lipoxygenase activities and the results were compared with standard aspirin 100 microg mL.Results The results showed dose dependent activity in all the three in vitro models but showed non-significant variation among the isolated alpha amyrin from two different plants.Conclusion Amount of isolated alpha amyrin was higher in Fb source 0.42 mg than Ab plant 0.23 mg and hence Fb was identified as a better source for isolation of alpha amyrin for further investigations.