Abstract
Aim. Comparison analysis of expression levels of Ki-67, p53, and caspase 3 was performed between the tumor cells and the stromal cells of non-invasive intestinal-type gastric adenocarcinoma on the one hand and the tumor cells and the stromal cells of invasive intestinal-type gastric adenocarcinoma on the other hand.Materials and methods. For this purpose, pathohistological and immunohistochemical studies were used. The surgical material of 10 patients with non-invasive (pTis, pT1) intestinal-type gastric adenocarcinoma and 10 patients with invasive (pT2, pT3, pT4) intestinal-type gastric adenocarcinoma were analyzed.Results. It was established that intestinal-type gastric adenocarcinoma is characterized by the medium Ki-67 labeling index, the low expression level of p53, the low expression level of caspase 3 in the tumor cells, and also the low Ki-67 labeling index and the extremely low expression level of caspase 3 in the stromal cells. The tumor cells of invasive intestinal-type gastric adenocarcinoma are characterized by the 2 times higher expression level of p53, than the tumor cells of non-invasive intestinal-type gastric adenocarcinoma. Moreover, direct correlation between the medium Ki-67 labeling index of the tumor cells and the low Ki-67 labeling index of the stromal cells, and also direct correlation between the medium Ki-67 labeling index in the tumor cells of intestinal-type gastric adenocarcinoma and the low expression level of p53 in these cells were identified.Conclusions. These data demonstrate a significantly more activity of proliferation and apoptosis in the tumor cells of intestinal-type gastric adenocarcinoma, in comparison with the stromal cells of this tumor. Invasive intestinal-type gastric adenocarcinoma differs by the 2 times higher expression level of p53, in comparison with the same index for non-invasive carcinoma. Furthermore, a tight association between proliferative processes in the tumor cells and in the stromal cells of intestinal-type gastric adenocarcinoma, and also an association between proliferative processes and accumulation of mutant protein p53 in the tumor cells of intestinal-type gastric adenocarcinoma were identified.
Highlights
Comparative immunohistochemical characteristic of expression levels of Ki-67, p53, caspase 3 in non-invasive and invasive intestinal-type gastric adenocarcinoma
Immunohistochemical studies results showed that nuclear expression of Ki-67 was determined in the tumor cells and the stromal cells of intestinal-type gastric adenocarcinoma, nuclear expression of p53 was determined in the tumor cells only and nuclear-cytoplasmic expression of caspase 3 was determined in the tumor cells and the stromal cells of this tumor
The tumor cells of non-invasive and invasive intestinal type gastric adenocarcinoma are characterized by the medium proliferation index, the low p53 expression level and the low caspase 3 expression level, while the stromal cells of this tumor are characterized by the low proliferation index and the extremely low caspase 3 expression level (p0.05)
Summary
Pathohistological and immunohistochemical study of non-invasive (pTis, pT1) intestinal-type gastric adenocarcinoma (10 patients, the age of the patients ranged from 45 to 83 years) and invasive (pT2, pT3, pT4) intestinal-type gastric adenocarcinoma (10 patients, the age of the patients ranged from 58 to 72 years) was performed. The expression of caspase 3 by the tumor cells and the stromal cells of the intestinal-type gastric adenocarcinoma was determined by the digital morphometry method [15]. In this way, the expression level of caspase 3 was expressed in the conventional units of the optical density (CUOD) and was graduated on 4 levels: lack of expression – 0–20 CUOD, the low expression level of caspase 3 – 21–50 CUOD, the moderate – 51–100 CUOD and the high expression level of caspase 3 – more than 100 CUOD. The study of correlations between the studied parameters was performed using Spearman’s rank correlation test
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