Comparative immunhistological examination of inner ear in wild type and pituitary adenylate cyclase activating polypeptide (PACAP) deficient mice

Abstract

Event Abstract Back to Event Comparative immunhistological examination of inner ear in wild type and pituitary adenylate cyclase activating polypeptide (PACAP) deficient mice A. Tamás1, B. Fülöp1, D. Reglődi1, P. Kiss1, R. Brubel1, G. Horváth1*, A. Lubics1, R. Gábriel2 and K. Szabadfi2 1 University of Pécs, Department of Anatomy, Medical School, Institute of Biology, Hungary 2 University of Pécs, Department of Experimental Zoology and Neurobiology, Institute of Biology, Hungary PACAP is a multifunctional neuropeptide with well known neuroprotective and neurotrophic effects. The involvement of PACAP in sensory processing has also been documented, but little is known about its effects in the auditory system. PACAP and its specific receptor (PAC1), are present in the cochlea and in brain structures involved in auditory pathways. Recently, we have shown that PACAP protects cochlear cells against oxidative stress-induced apoptosis. The endolymphatic Ca2+ cc. controlled by Ca2+ buffers of the hair cells is essential for the normal hearing processes. In this study we examined the localization of PAC1-receptor and Ca2+ buffering proteins (parvalbumin, calretinin) in the inner ear of 5-day-old PACAP deficient mice compared with wild type mice to investigate the effect of endogenous PACAP in the cochlear function. We did not find difference in the distribution of PAC1 receptors between groups. In contrast, inner and outer hair cells of PACAP KO mice showed more pronounced parvalbumin and calretinin immunpositivity compared with wild type mice. Elevated endolymphatic Ca2+ is deleterious for the cochlear function, against which the high cc. of Ca2+ buffers in hair cells may protect. Meanwhile, the increased immunreactivity of Ca2+ binding proteins in the absence of PACAP provide further evidence the important role of PACAP in the hearing processes. Acknowledgements Support: OTKAK72592, F67830, CNK78480, ETT278-04/2009, Bolyai Scholarship,Richter Foundation Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Tamás A, Fülöp B, Reglődi D, Kiss P, Brubel R, Horváth G, Lubics A, Gábriel R and Szabadfi K (2011). Comparative immunhistological examination of inner ear in wild type and pituitary adenylate cyclase activating polypeptide (PACAP) deficient mice. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00053 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. G. Horváth, University of Pécs, Department of Anatomy, Medical School, Institute of Biology, Pécs, Hungary, horvath@phys.szote.u-szeged.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers A. Tamás B. Fülöp D. Reglődi P. Kiss R. Brubel G. Horváth A. Lubics R. Gábriel K. Szabadfi Google A. Tamás B. Fülöp D. Reglődi P. Kiss R. Brubel G. Horváth A. Lubics R. Gábriel K. Szabadfi Google Scholar A. Tamás B. Fülöp D. Reglődi P. Kiss R. Brubel G. Horváth A. Lubics R. Gábriel K. Szabadfi PubMed A. Tamás B. Fülöp D. Reglődi P. Kiss R. Brubel G. Horváth A. Lubics R. Gábriel K. Szabadfi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.