Abstract
Immobilization of polyclonal antibodies was studied on native screen-printed graphite electrodes (SPEs) and variously modified electrodes. SPEs coated with didodecylammonium bromide (DDAB, a synthetic membranelike substance) films with gold nanoparticles gave the maximum electrochemical response. DDAB and gold nanoparticle films strongly changed the surface morphology, and the electrochemical signal became more intense and stable. This immobilization method increased the concentration of immobilized antibodies while their activity was retained. The detection limit of the enzymatic label (horseradish peroxidase) was 0.02 ng/L of sample.
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