Comparative Histological Study on the Effect of Stem Cells, and Gene Modified Stem Cells in Experimentally-Induced Diabetes Type 1 Cardiomyopathy

Abstract

Background and Objectives: Sarco(endo) plasmic reticulum Ca2+-ATPase (SERCA2a) is the ATP-driven pump thattranslocates Ca2+ from the cytoplasm to the lumen of the sarcoplasmic reticulum. 25% of children and adolescents withtype 1 diabetes will develop diastolic dysfunction that results in part from a reduction in the activity of SERCA2a. Thepresent study aimed at investigating and comparing the therapeutic effect of Adipose Mesenchymal Stem Cells (AMSCs)with SERCA2a gene modified AMSCs in diabetes type 1 induced cardiomyopathy of adult male albino rat.Materials and Methods: Twenty-nine adult male albino rats were divided into: Donor group of 2 rats used to obtainAMSCs. Group I (Control group): 6 rats not exposed to diabetes induction. Group II (Diabetic group): 7 rats injectedwith streptozotocin (STZ) 50mg/Kg once. Group III ( (AMSCs group): 7 rats injected with AMSCs following inductionof diabetes. Group IV (SERCA2a Modified AMSCs Group): 7 rats injected with SERCA2a modified AMSCs, followinginduction of diabetes. All rats were sacrificed 8 weeks from start of experiment.Results: Morphological changes, indicating inflammation and degeneration, were found in the cardiac muscle of diabeticrats and regressed remarkably by AMSCs and SERCA2a modified AMSCs. The regression of morphological changes wasconfirmed by histological, immunohistochemical, morphometric and serological studies.Conclusion: The therapeutic effect of SERCA2a modified AMSCs in diabetes type 1 cardiomyopathy was moreremarkable than that of AMSCs.