Comparative histochemical observations on the excretory system of helminth parasites

Abstract

The excretory canals of Ascaridia galli (Nematoda) and the protonephridial ducts of Cotylophoron cotylophorum (Trematoda) and Raillietina cesticillus (Cestoda) have been studied with regard to the histochemical localization of lipids, carbohydrates and hydrolytic enzymes. Distinct excretory organs are absent in the acanthocephalan Centrorhynchus corvi. Triglycerides, phospholipids and lipoproteins are seen in association with the wall of excretory canals of A. galli and R. cesticillus, and phospholipids and lipoproteins at the corresponding site in C. cotylophorum. The physiological significance of lipids in association with excretion of substances has been discussed. Low molecular weight glycogen is present in the lumen of excretory canal of A. galli but not in other worms. The common feature of the excretory canals is the presence of enzyme activities of nonspecific alkaline phosphatase and Mg2+-dependent ATPase. Activity of acid phosphatase is seen only in the excretory canals of A. galli. Glucose-6-phosphatase is present in A. galli and C. cotylophorum and absent in R. cesticillus. Weak reaction of 5'-nucleotidase is present in the excretory canals of helminth species studied here. The role of these enzymes in transportation of substances across the wall of excretory canals and also in ionic regulation has been discussed in detail.