Abstract
BackgroundPectobacterium carotovorum subsp. brasiliense is a broad host range bacterial pathogen, which causes blackleg of potatoes and bacterial soft rot of vegetables worldwide. Production of plant cell wall degrading enzymes is usually critical for Pectobacterium infection. However, other virulence factors and the mechanisms of genetic adaptation still need to be studied in detail.ResultsIn this study, the complete genome of P. carotovorum subsp. brasiliense strain SX309 isolated from cucumber was compared with eight other pathogenic bacteria belonging to the Pectobacterium genus, which were isolated from various host plants. Genome comparison revealed that most virulence genes are highly conserved in the Pectobacterium strains, especially for the key virulence determinants involved in the biosynthesis of extracellular enzymes and others including the type II and III secretion systems, quorum sensing system, flagellar and chemotactic genes. Nevertheless, some variable regions of the T6SS and the CRISP-Cas immune system are unique for P. carotovorum subsp. brasiliense.ConclusionsThe extensive comparative genomics analysis revealed highly conserved virulence genes in the Pectobacterium strains. However, several variable regions of type VI secretion system and two subtype Cas mechanism-Cas immune systems possibly contribute to the process of Pectobacterium infection and adaptive immunity.
Highlights
Pectobacterium carotovorum subsp. brasiliense is a broad host range bacterial pathogen, which causes blackleg of potatoes and bacterial soft rot of vegetables worldwide
Li et al BMC Genomics (2019) 20:486 carotovorum subsp. brasiliense BC1 isolated from Brassica rapa ssp. pekinensis [2] and P. carotovorum subsp. brasiliense BZA12 isolated from Cucumis sativus [9], P. carotovorum subsp. odoriferum BC S7 isolated from Brassica rapa ssp. pekinensis
We found that the genes encoding Plant Cell Wall Degrading Enzymes (PCWDE), T2SS, T3SS, T6SS, quorum sensing (QS) system, two-component system and LPS are probably major virulence factors, and the Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated sequence (Cas) system may be involved in adaptive immunity
Summary
Pectobacterium carotovorum subsp. brasiliense is a broad host range bacterial pathogen, which causes blackleg of potatoes and bacterial soft rot of vegetables worldwide. Brasiliense is a broad host range bacterial pathogen, which causes blackleg of potatoes and bacterial soft rot of vegetables worldwide. Li et al BMC Genomics (2019) 20:486 carotovorum subsp. Brasiliense BC1 isolated from Brassica rapa ssp. Pekinensis [2] and P. carotovorum subsp. Brasiliense BZA12 isolated from Cucumis sativus [9], P. carotovorum subsp. Odoriferum BC S7 isolated from Brassica rapa ssp. Brasiliense was originally reported in Brazil and has since been fully described [10]. During 2014-2016, a devastating cucumber bacterial soft rot caused by P. carotovorum subsp. Very few studies have been focused on studying the complete genome of P. carotovorum subsp. Brasiliense, and the pathogenicity and the genetic adaptation to the host of this subspecies remain largely unknown Very few studies have been focused on studying the complete genome of P. carotovorum subsp. brasiliense, and the pathogenicity and the genetic adaptation to the host of this subspecies remain largely unknown
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