Abstract
Plant growth-promoting rhizobacteria (PGPR) are a group of rhizosphere bacteria that promote plant growth. Delftia tsuruhatensis MTQ3 is a member of PGPR that produces siderophores. The draft genome sequence of MTQ3 has been reported. Here, we analyzed the genome sequence of MTQ3 and performed a comparative genome analysis of four sequenced Delftia strains, revealing genetic relationships among these strains. In addition, genes responsible for bacteriocin and nonribosomal peptide synthesis were detected in the genomes of each strain. To reveal the functions of NRPS genes in siderophore production in D. tsuruhatensis MTQ3, three NRPS genes were knocked out to obtain the three mutants MTQ3-Δ1941, MTQ3-Δ1945, and MTQ3-Δ1946, which were compared with the wild-type strain. In qualitative and quantitative analyses using CAS assay, the mutants failed to produce siderophores. Accordingly, the NRPS genes in MTQ3 were functionally related to siderophore production. These results clarify one mechanism by which plant growth is promoted in MTQ3 and have important applications in agricultural production.
Highlights
Plant growth-promoting rhizobacteria (PGPR) could promote plant growth by a variety of mechanisms, such as siderophore production [1], antibiotics secretion, phytohormone generation, and the induction of systemic resistance [2, 3]
These results suggested that strain MTQ3 had high homology with D. tsuruhatensis
D. tsuruhatensis MTQ3 was examined as a PGPR
Summary
Plant growth-promoting rhizobacteria (PGPR) could promote plant growth by a variety of mechanisms, such as siderophore production [1], antibiotics secretion, phytohormone generation, and the induction of systemic resistance [2, 3]. Species in the genus Delftia have been described as PGPR [10], their ability to produce siderophores has not been verified. The draft genome sequence of MTQ3 was formerly reported [11], but the mechanism of siderophore production is unclear. A comparative genomic analysis of MTQ3 and the related genome sequences of Delftia sp. Wild-type strain, Rifr, Kms D. tsuruhatensis MTQ3−Δ1941, Rifr, Kmr D. tsuruhatensis MTQ3−Δ1945, Rifr, Kmr D. tsuruhatensis MTQ3−Δ1946, Rifr, Kmr. Suicide plasmid carrying sacB, Gmr, selecting double crossover MTQ3 pJQ200SK carrying Km and the fragment of orf-1941, Gmr, Kmr pJQ200SK carrying Km and the fragment of orf-1945, Gmr, Kmr pJQ200SK carrying Km and the fragment of orf-1946, Gmr, Kmr Carrying Km cassette (Pst I), Kmr Helper plasmid used in triparental mating, Kmr, Rifs TA cloning vector, Ampr. We constructed NRPS gene knockout mutants to analyze gene function with respect to siderophore biosynthesis
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