Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes.

Ahmed M Moustafa,Ben Adler,Torsten Seemann,Simon Gladman,Mark D Bennett,John D Boyce,Marina Harper,Jingfa Xiao

doi:10.1371/journal.pone.0130296

Ahmed M Moustafa, Ben Adler + Show 6 more

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https://doi.org/10.1371/journal.pone.0130296

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Abstract

Pasteurella multocida is the primary causative agent of a range of economically important diseases in animals, including haemorrhagic septicaemia (HS), a rapidly fatal disease of ungulates. There is limited information available on the diversity of P. multocida strains that cause HS. Therefore, we determined draft genome sequences of ten disease-causing isolates and two vaccine strains and compared these genomes using a range of bioinformatic analyses. The draft genomes of the 12 HS strains were between 2,298,035 and 2,410,300 bp in length. Comparison of these genomes with the North American HS strain, M1404, and other available P. multocida genomes (Pm70, 3480, 36950 and HN06) identified a core set of 1,824 genes. A set of 96 genes was present in all HS isolates and vaccine strains examined in this study, but absent from Pm70, 3480, 36950 and HN06. Moreover, 59 genes were shared only by the Asian B:2 strains. In two Pakistani isolates, genes with high similarity to genes in the integrative and conjugative element, ICEPmu1 from strain 36950 were identified along with a range of other antimicrobial resistance genes. Phylogenetic analysis indicated that the HS strains formed clades based on their country of isolation. Future analysis of the 96 genes unique to the HS isolates will aid the identification of HS-specific virulence attributes and facilitate the development of disease-specific diagnostic tests.

Highlights

Pasteurella multocida is a Gram-negative, nonmotile, nonspore-forming coccobacillus. It is the causative agent of a spectrum of economically important diseases worldwide, including atrophic rhinitis in pigs, haemorrhagic septicaemia (HS) in cattle and buffalo, fowl cholera in poultry, snuffles in rabbits and sporadic human infections that often follow dog or cat bites [1,2]
P. multocida is a heterogeneous species with strains being commonly differentiated by serology [3], or more recently capsular locus-specific multiplex PCR [4], into five capsular serogroups designated A, B, D, E and F
Ten P. multocida strains, isolated from HS cases in buffalo (8 isolates) and cattle (2 isolates) from Pakistan and Thailand, and two Pakistani HS vaccine strains were used for this study (Table 1)

Summary

Introduction

Pasteurella multocida is a Gram-negative, nonmotile, nonspore-forming coccobacillus. It is the causative agent of a spectrum of economically important diseases worldwide, including atrophic rhinitis in pigs, haemorrhagic septicaemia (HS) in cattle and buffalo, fowl cholera in poultry, snuffles in rabbits and sporadic human infections that often follow dog or cat bites [1,2]. A second serological typing system is often used to differentiate strains into 16 Heddleston serotypes or serovars based on lipopolysaccharide (LPS) antigens [5]. Full strain designations usually combine both systems, such that a designation of B:2 indicates capsule serogroup B and LPS serovar 2

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