Abstract
Mycobacterium cosmeticum is a nontuberculous Mycobacterium recovered from different water sources including household potable water and water collected at nail salon. Individual cases of this bacterium have been reported to be associated with gastrointestinal tract infections. Here we present the first whole-genome study and comparative analysis of two new clinically-derived Mycobacterium sp. UM_RHS (referred as UM_RHS after this) and Mycobacterium sp. UM_NYF (referred as UM_NYF after this) isolated from patients in Indonesia and Malaysia respectively to have a better understanding of the biological characteristic of these isolates. Both strains are likely Mycobacterium cosmeticum as supported by the evidence from molecular phylogenetic, comparative genomic and Average Nucleotide Identity (ANI) analyses. We found the presence of a considerably large number of putative virulence genes in the genomes of UM_RHS and UM_NYF. Interestingly, we also found a horizontally transferred genomic island carrying a putative dsz operon proposing that they may have potential to perform biodesulfization of dibenzothiophene (DBT) that may be effective in cost reduction and air pollution during fuel combustion. This comparative study may provide new insights into M. cosmeticum and serve as an important reference for future functional studies of this bacterial species.
Highlights
Mycobacterium is a genus under the actinobacteria phylum classified together with other wellknown human pathogens like M. tuberculosis and M. leprae [1,2,3]
This genus consists of another group of mycobacteria known as the nontuberculous mycobacteria (NTM)
39,529,963 preprocessed reads were used for assembly
Summary
Mycobacterium is a genus under the actinobacteria phylum classified together with other wellknown human pathogens like M. tuberculosis (causing tuberculosis) and M. leprae (causing leprosy) [1,2,3]. We constructed a 16S rRNA-based phylogenetic tree using UM_RHS, UM_NYF and other mycobacterial species (Fig 1). Both UM_RHS and UM_NYF genomes generally share similar genomic features such as the genome size, number of protein-coding genes and RNA as predicted by the Rapid Annotation using Subsystem Technology (RAST) pipeline [20].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
