Abstract
Arthrobacter is one of the most prevalent genera of nicotine-degrading bacteria; however, studies of nicotine degradation in Arthrobacter species remain at the plasmid level (plasmid pAO1). Here, we report the bioinformatic analysis of a nicotine-degrading Arthrobacter aurescens M2012083, and show that the moeB and mogA genes that are essential for nicotine degradation in Arthrobacter are absent from plasmid pAO1. Homologues of all the nicotine degradation-related genes of plasmid pAO1 were found to be located on a 68,622-bp DNA segment (nic segment-1) in the M2012083 genome, showing 98.1% nucleotide acid sequence identity to the 69,252-bp nic segment of plasmid pAO1. However, the rest sequence of plasmid pAO1 other than the nic segment shows no significant similarity to the genome sequence of strain M2012083. Taken together, our data suggest that the nicotine degradation-related genes of strain M2012083 are located on the chromosome or a plasmid other than pAO1. Based on the genomic sequence comparison of strain M2012083 and six other Arthrobacter strains, we have identified 17 σ70 transcription factors reported to be involved in stress responses and 109 genes involved in environmental adaptability of strain M2012083. These results reveal the molecular basis of nicotine degradation and survival capacities of Arthrobacter species.
Highlights
Arthrobacter is one of the most prevalent genera of nicotine-degrading bacteria; studies of nicotine degradation in Arthrobacter species remain at the plasmid level
Nicotine is a typical N-heterocyclic pollutant and Arthrobacter is one of the most prevalent genera of nicotine-degrading bacteria, studies of nicotine degradation by Arthrobacter species remain at the plasmid level, while the complete genome of another prevalent bacterium, Pseudomonas putida S16, has been sequenced and extensively studied[37,38,39,40,41,42,43]
The nicotine-degrading strain M2012083 was isolated from tobacco waste and deposited in the China Center for Type Culture Collection (CCTCC; collection number M2012083)
Summary
Arthrobacter is one of the most prevalent genera of nicotine-degrading bacteria; studies of nicotine degradation in Arthrobacter species remain at the plasmid level (plasmid pAO1). Based on the genomic sequence comparison of strain M2012083 and six other Arthrobacter strains, we have identified 17 s70 transcription factors reported to be involved in stress responses and 109 genes involved in environmental adaptability of strain M2012083 These results reveal the molecular basis of nicotine degradation and survival capacities of Arthrobacter species. Some N-heterocyclic pollutant-degrading Arthrobacter species such as the s-triazine-degrading strain A. aurescens TC1 (TC1) and quinaldine-degrading strain Arthrobacter nitroguajacolicus Rue61a (Rue61a) have been isolated from atrazine-containing soil and the sewage sludge of a coal tar refining factory, respectively[18,31,32] Their complete genomes have been sequenced, and the molecular bases of their metabolic and survival potentials have been thoroughly analyzed[27,33]. It is necessary to obtain and analyze the genome sequence of a nicotine-degrading Arthrobacter strain in order to uncover the molecular basis of its survival capacity
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